Difference between revisions of "Part:BBa K314201:Experience"

(Applications of BBa_K314201)
(Applications of BBa_K314201)
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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'''Evidence that Tsi2 works as expected can be found on [http://2010.igem.org/Team:Washington/Gram_Negative/Test this page] from the 2010 UW iGEM Wiki, under the heading "Evidence that Tse2 and Tsi2 are Function As Expected"'''
  
 
===Applications of BBa_K314201===
 
===Applications of BBa_K314201===
If [https://parts.igem.org/wiki/index.php?title=Part:BBa_K314200 Tse2] is combined with a functioning Type 6 secretion system and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K314201 Tsi2],it results in a killing system that can be targeted to gram negative bacteria. This could be used in a probiotic application.
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Tsi2 is necessary for the generation of Gram-negative bacteria that have been directed to target other Gram-negative bacteria for killing by [https://parts.igem.org/Part:BBa_K314200 Tse2] in response to a user-defined signal.  Tsi2 is necessary because it protects host cells from death caused by the Tse2 toxic protein.
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If the antitoxin Tsi2 is combined with a functioning Type 6 Secretion System and the toxin [https://parts.igem.org/wiki/index.php?title=Part:BBa_K314200 Tse2] it results in a killing system that can be targeted to Gram-negative bacteria. This could be used in a probiotic application.  This was the goal behind the Gram-negative portion of the [http://2010.igem.org/Team:Washington 2010 UW iGEM project].
  
 
===User Reviews===
 
===User Reviews===

Revision as of 23:10, 21 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Evidence that Tsi2 works as expected can be found on [http://2010.igem.org/Team:Washington/Gram_Negative/Test this page] from the 2010 UW iGEM Wiki, under the heading "Evidence that Tse2 and Tsi2 are Function As Expected"

Applications of BBa_K314201

Tsi2 is necessary for the generation of Gram-negative bacteria that have been directed to target other Gram-negative bacteria for killing by Tse2 in response to a user-defined signal. Tsi2 is necessary because it protects host cells from death caused by the Tse2 toxic protein.

If the antitoxin Tsi2 is combined with a functioning Type 6 Secretion System and the toxin Tse2 it results in a killing system that can be targeted to Gram-negative bacteria. This could be used in a probiotic application. This was the goal behind the Gram-negative portion of the [http://2010.igem.org/Team:Washington 2010 UW iGEM project].

User Reviews

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