Difference between revisions of "Part:BBa K314200:Experience"

Revision as of 23:03, 21 October 2010

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Applications of BBa_K314200

Tse2 is a potent bacterial toxin and thus has many applications, with or without its immunity protein, Tsi2. One potential application is use as a biotechnology agent in the way that the CcdB, the Death Protein, is used.

Tse2 could also be combined with the T6SS to result in a system that targets this toxin into the cytoplasm of target Gram-negative bacteria. This is useful to create Gram-negative bacteria that have been directed to target other Gram-negative bacteria for killing in response to a user-defined signal.

If Tse2 is combined with a functioning Type 6 Secretion System and the antitoxin Tsi2 it results in a killing system that can be targeted to Gram-negative bacteria. This could be used in a probiotic application. This was the goal behind the Gram-negative portion of the [http://2010.igem.org/Team:Washington 2010 UW iGEM project].

Diagram of the Tse2/Tsi2/ Type VI Secretion System as it would be used in a probiotic application

User Reviews

UNIQ6ec440a8b8f3bc32-partinfo-00000000-QINU UNIQ6ec440a8b8f3bc32-partinfo-00000001-QINU

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 ===Applications of BBa_K314200===
-If Tse2 is combined with a functioning Type 6 Secretion System and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K314201 Tsi2] it results in a killing system that can be targeted to gram negative bacteria. This could be used in a probiotic application.Tse2 could also be used as a death gene similar to ccdb.
+Tse2 is a potent bacterial toxin and thus has many applications, with or without its immunity protein, [https://parts.igem.org/Part:BBa_K314201 Tsi2].  One potential application is use as a biotechnology agent in the way that the CcdB, the Death Protein, is used.
+Tse2 could also be combined with the T6SS to result in a system that targets this toxin into the cytoplasm of target Gram-negative bacteria.  This is useful to create Gram-negative bacteria that have been directed to target other Gram-negative bacteria for killing in response to a user-defined signal.
+If Tse2 is combined with a functioning Type 6 Secretion System and the antitoxin [https://parts.igem.org/wiki/index.php?title=Part:BBa_K314201 Tsi2] it results in a killing system that can be targeted to Gram-negative bacteria. This could be used in a probiotic application.  This was the goal behind the Gram-negative portion of the [http://2010.igem.org/Team:Washington 2010 UW iGEM project].
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