Difference between revisions of "Part:BBa K398000"
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LadA's ability to preferentially capture long-chain alkanes for oxidation sets it apart from other flavoprotein monooxygenases[1].
 
LadA's ability to preferentially capture long-chain alkanes for oxidation sets it apart from other flavoprotein monooxygenases[1].
  
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[[Image:LadA-dimer.jpg|500px|thumb|left|1% agarose of colony PCR. Gel run at 100V for 50 minutes. Of all samples 5 µL was loaded with 1 µL loadingbuffer. 5 µL was loaded of marker]]
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[[Image:LadA-Dimer.jpg|500px|thumb|right|Ribbon diagram of the LadA dimer.]]
 
   
 
   
  

Revision as of 09:54, 21 October 2010

Long-Chain Alkane Monooxygenase

A flavoprotein alkane monooxygenase native to Geobacillus thermodinitrificans NG-80-2. It has been found to specifically oxidize the terminal regions of alkanes ranging from C15 up to at least C36. The product is the corresponding primary alkanol. LadA forms a catalytic complex with flavin mononucleotide (FMN) which utilizes dioxygen to insert an oxygen atom into the substrate.

The general catalytic function involves three chemical processes:

  • Reduction of the cofactor flavin mononucleotide (FMN to FMNH2) by NAD(P)H
  • Reaction of FMNH2 with O2
  • Binding, orienting, and activating the substrate for its oxygenation

LadA's ability to preferentially capture long-chain alkanes for oxidation sets it apart from other flavoprotein monooxygenases[1].

Ribbon diagram of the LadA dimer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 908