Difference between revisions of "Part:BBa K319014:Design"
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| + | Ellis T, Wang X and Collins JJ. Diversity-based, model-guided construction of synthetic gene networks with predicted functions. Nature Biotechnology 27: 465-471 (2009). | ||
Latest revision as of 03:23, 21 October 2010
yeast T13 Proximal Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 115
Design Notes
The part was designed using primers that introduced standard 20 bp primer binding sites flanking the upstream and downstream regions of the part.
Source
The part was amplified via colony PCR from a YPH500 strain of S. cerevisiae. The strain expressing the part was given to us by Tom Ellis.
References
Ellis T, Wang X and Collins JJ. Diversity-based, model-guided construction of synthetic gene networks with predicted functions. Nature Biotechnology 27: 465-471 (2009).