Difference between revisions of "Part:BBa K314203"
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Tse2/Tsi2 w/HSL inducible promoter
 
Tse2/Tsi2 w/HSL inducible promoter
  
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This part contains  part F2620 ( the LuxR
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This part contains  the Tse2/Tsi2 locus from ''Pseudomonas aeruginosa'' downstream from an HSL induced promoter( F2620)
 
===Usage and Biology===
 
===Usage and Biology===
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Tse2 is a toxin that can be exported into  gram (-) bacteria by the Type VI secretion system, killing all gram (-) cells not expressing Tsi2 ( the antitoxin of Tse2). Since Tse2 is being coexpressed with Tsi2, cells transformed with this part will not  die.  Therefore, this part is useful for regulating secretion of Tse2.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K314203 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K314203 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
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===Functional Parameters===
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===Functional Parameters===  
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This part has been tested in pSB3k3. ''E. coli'' transformed with this part showed Tse2 production in a broth culture containing HSL at a concentration of 10µM showed Tse2 expression, while no appreciable level of Tse2 was produced in an environment not containing HSL. This was verified by an anti-Tse2 western blot   
 
<partinfo>BBa_K314203 parameters</partinfo>
 
<partinfo>BBa_K314203 parameters</partinfo>
 
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Revision as of 00:15, 21 October 2010

HSL inducible toxin antitoxin, Tse2 + Tsi2

Tse2/Tsi2 w/HSL inducible promoter

This part contains the Tse2/Tsi2 locus from Pseudomonas aeruginosa downstream from an HSL induced promoter( F2620)

Usage and Biology

Tse2 is a toxin that can be exported into gram (-) bacteria by the Type VI secretion system, killing all gram (-) cells not expressing Tsi2 ( the antitoxin of Tse2). Since Tse2 is being coexpressed with Tsi2, cells transformed with this part will not die. Therefore, this part is useful for regulating secretion of Tse2. !-- --> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal NgoMIV site found at 1340
    Illegal NgoMIV site found at 1465
    Illegal NgoMIV site found at 1795
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal SapI.rc site found at 1790