Difference between revisions of "Part:BBa K314200:Design"

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===Design Notes===
 
===Design Notes===
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mention something about promoter needs to have no leaky expression for succesful cloning
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When planning on expressing Tse2 a promoter with no or very little leaky expression must be used. We were only able to obtain non-functioning mutants of the protein when we attempted to biobrick Tse2 with the F2620 promoter.
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===Source===
 
===Source===

Revision as of 23:57, 20 October 2010

Toxin Tse2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 196
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 378
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When planning on expressing Tse2 a promoter with no or very little leaky expression must be used. We were only able to obtain non-functioning mutants of the protein when we attempted to biobrick Tse2 with the F2620 promoter.

Source

pseudomonas aeruginosa

References

Cell Host Microbe.�2010 Jan 21;7(1):25-37