Difference between revisions of "Part:BBa K398014"

Revision as of 20:43, 20 October 2010

Alkane Hydroxylase System

The alkane hydroxylase system from Gordonia sp. TF6 facilitates the initial step of the degradation of C5-C13 alkanes as well as that of C5-C8 cycloalkanes. The cluster is formed by the genes for alkB2 (alkane 1-monooxygenase), rubA3 (rubredoxin), rubA4 (rubredoxin) and rubB (rubredoxin reductase). alkB2 is a non-haem diiron monooxygenase membrane protein. This monooxygenase oxidizes (cyclo)alkanes to the respective (cyclo)alkanols, requires two soluble electron-carrier proteins, rubredoxin (rubA3 & rubA4) and rubredoxin reductase (rubB).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K398014 short</partinfo>
-The alkB gene cluster from Gordonia sp. TF6 facilitates the initial step of the degradation of C5-13 alkanes as well as that of C5-8 cycloalkanes. The cluster is formed by the genes for alkB2 (alkane 1-monooxygenase), rubA3 (rubredoxin), rubA4 (rubredoxin) and rubB (rubredoxin reductase). alkB2 is a non-haem diiron monooxygenase membrane protein. This monooxygenase oxidizes n-alkanes to the respective n-alkanols and requires three soluble electron-transfer proteins, rubredoxin (rubA3 & rubA4) and rubredoxin reductase (rubB).
+The alkane hydroxylase system from Gordonia sp. TF6 facilitates the initial step of the degradation of C5-C13 alkanes as well as that of C5-C8 cycloalkanes. The cluster is formed by the genes for alkB2 (alkane 1-monooxygenase), rubA3 (rubredoxin), rubA4 (rubredoxin) and rubB (rubredoxin reductase). alkB2 is a non-haem diiron monooxygenase membrane protein. This monooxygenase oxidizes (cyclo)alkanes to the respective (cyclo)alkanols, requires two soluble electron-carrier proteins, rubredoxin (rubA3 & rubA4) and rubredoxin reductase (rubB).
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