Difference between revisions of "Part:BBa K358019:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K358019===
 
===Applications of BBa_K358019===
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Using this part, we checked the function of SRRz gene.
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Firstly, we constructed this part on low copy plasmid, pSB4K5, and transformed into KRX.
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It is necessary to repress the lactose promoter and avoid the disadvantage of cell lysis.
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Secondly, we cultured E.coli on M9-Km overnight. 
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Experiment 1:
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Then, diluted the sample and added IPTG as the inducer.  We measured A550 at each time.
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Experiment 2:
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We added IPTG, not dilute the sample.  We measured A550 at each time.
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Finally,
  
 
===User Reviews===
 
===User Reviews===

Revision as of 20:33, 19 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K358019

Using this part, we checked the function of SRRz gene.

Firstly, we constructed this part on low copy plasmid, pSB4K5, and transformed into KRX. It is necessary to repress the lactose promoter and avoid the disadvantage of cell lysis.

Secondly, we cultured E.coli on M9-Km overnight.

Experiment 1: Then, diluted the sample and added IPTG as the inducer. We measured A550 at each time.


Experiment 2: We added IPTG, not dilute the sample. We measured A550 at each time.


Finally,

User Reviews

UNIQ1361614d9fd36bdb-partinfo-00000000-QINU UNIQ1361614d9fd36bdb-partinfo-00000001-QINU