Difference between revisions of "Part:BBa K358019"

Revision as of 20:07, 19 October 2010

Lysis cassette regulated by lacP

Lysis cassette[SRRz] is regulated by lactose promoter. Activating lactose promoter and expressing SRRz gene, it causes the cell lysis. So, lactose promoter must be repressed when transform this part.

To repress lactose promoter tightly, we constructed this part on low copy plasmid, pSB4K5, and transformed into KRX, not Top 10. KRX has lacI in its genome and Top 10 hasn't (see genotype[http://www.promega.com/pnotes/94/14410_27/14410_27.pdf],[http://openwetware.org/wiki/E._coli_genotypes#TOP10_.28Invitrogen.29]).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K358019 short</partinfo>
-Lysis cassette[SRRz] is regulated by lactose promoter.  Activate lactose promoter and express SRRz gene, it causes the cell lysis.
+Lysis cassette[SRRz] is regulated by lactose promoter.
+Activating lactose promoter and expressing SRRz gene, it causes the cell lysis.  So, lactose promoter must be repressed when transform this part.
+To repress lactose promoter tightly, we constructed this part on low copy plasmid, pSB4K5, and transformed into KRX, not Top 10.  KRX has lacI in its genome and Top 10 hasn't (see genotype[http://www.promega.com/pnotes/94/14410_27/14410_27.pdf],[http://openwetware.org/wiki/E._coli_genotypes#TOP10_.28Invitrogen.29]).
 <!-- Add more about the biology of this part here