Difference between revisions of "Part:BBa K358021:Design"

 
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===Design Notes===
 
===Design Notes===
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Lactose promoter must be repressed, even though the anti-killergene is expressed constitutively.  So, we used low copy plasmid, pSB4K5, and transformed it to KRX, not Top 10.  KRX is able to repress the lactose promoter.  See KRX genotype[http://www.promega.com/pnotes/94/14410_27/14410_27.pdf].
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===Source===
 
===Source===

Revision as of 19:28, 19 October 2010

Lysisbox -type2-


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


Design Notes

Lactose promoter must be repressed, even though the anti-killergene is expressed constitutively. So, we used low copy plasmid, pSB4K5, and transformed it to KRX, not Top 10. KRX is able to repress the lactose promoter. See KRX genotype[http://www.promega.com/pnotes/94/14410_27/14410_27.pdf].

Source

lambda phage DNA and biobrick

References