Difference between revisions of "Part:BBa K358019:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
−
biobrick, lambda phage DNA
+
biobrick[[BBa_R0011]], lambda phage DNA
  
 
===References===
 
===References===

Revision as of 19:13, 19 October 2010

Lysis cassette regulated by lacP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because of the lysis cassette, lactose promoter must be repressed when transform it. So, we used low copy plasmid, pSB4K5, and then transformed it to KRX, not Top 10. It has lacI in the genome. See KRX genotype here, [http://www.promega.com/pnotes/94/14410_27/14410_27.pdf].

Source

biobrickBBa_R0011, lambda phage DNA

References