Difference between revisions of "Part:BBa K300091"
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<partinfo>BBa_K300091 short</partinfo> | <partinfo>BBa_K300091 short</partinfo> | ||
| − | This construct was used to test the possibility to induce the synthesis of fusion proteins composed by phasin (<partinfo>BBa_K300002</partinfo>), intein (<partinfo>BBa_K300004</partinfo>), linkers (<partinfo>BBa_K105012</partinfo>) and GFP reporter (<partinfo>BBa_K300005</partinfo>). With this BioBrick was also tested the right folding of the recombinant proteins | + | This construct was used to test the possibility to induce the synthesis of fusion proteins composed by phasin (<partinfo>BBa_K300002</partinfo>), intein (<partinfo>BBa_K300004</partinfo>), linkers (<partinfo>BBa_K105012</partinfo>) and GFP reporter (<partinfo>BBa_K300005</partinfo>). With this BioBrick was also tested the right folding of the recombinant proteins, but at the same time it can also be considered a device for purification of these proteins. |
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Revision as of 13:09, 19 October 2010
3OC6HSL inducible generator for BBa_K300095
This construct was used to test the possibility to induce the synthesis of fusion proteins composed by phasin (BBa_K300002), intein (BBa_K300004), linkers (BBa_K105012) and GFP reporter (BBa_K300005). With this BioBrick was also tested the right folding of the recombinant proteins, but at the same time it can also be considered a device for purification of these proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1004
Illegal BsaI.rc site found at 2859
Illegal SapI.rc site found at 2165