Difference between revisions of "Part:BBa I712019:Experience"

Revision as of 09:39, 18 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I712019

User Reviews

UNIQ2586c1a43bd0d9d9-partinfo-00000000-QINU

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theos

The Cambridge team confirmed that this part did emit light from E. coli when placed under an expression vector (:BBa_J13002) with the addition of D-luciferin. Despite the claim that the "part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix" we were able to express it in E. coli after adding a prokaryotic RBS and promoter using standard BioBrick assembly.

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UNIQ2586c1a43bd0d9d9-partinfo-00000002-QINU

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 <I>theos</I>
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-The Cambridge team confirmed that this part did emit light from E. coli when placed under an expression vector ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J13002 :BBa_J13002]) with the addition of D-luciferin.
+The Cambridge team confirmed that this part did emit light from E. coli when placed under an expression vector ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J13002 :BBa_J13002]) with the addition of D-luciferin.  Despite the claim that the "part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix" we were able to express it in E. coli after adding a prokaryotic RBS and promoter using standard BioBrick assembly.
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