Difference between revisions of "Part:BBa K411001:Design"

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* forward primer : 5' - gaattcgcggccgcttctagag ggtgataccagcatcgtcttgatgcccttggcag - 3'
 
* forward primer : 5' - gaattcgcggccgcttctagag ggtgataccagcatcgtcttgatgcccttggcag - 3'
 
* reverse primer : 5' - ctgcagcggccgctactagta cttgttgtcttgcagcggggtgctgccaagggcatcaagac - 3'
 
* reverse primer : 5' - ctgcagcggccgctactagta cttgttgtcttgcagcggggtgctgccaagggcatcaagac - 3'
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See [http://2010.igem.org/Team:NYMU-Taipei NYMU-Taipei's Design page]
 
  
 
===References===
 
===References===

Revision as of 08:38, 18 October 2010

Theophylline-inducible Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to choose a kind of riboswitch which has the following characteristics: The inducer cannot metabolize in E. coli. It does not exist in naturally occurring E. coli It does not have EcoRI,X,S, or PstI cutting site



Source

Synthesized directly from two primers:

  • forward primer : 5' - gaattcgcggccgcttctagag ggtgataccagcatcgtcttgatgcccttggcag - 3'
  • reverse primer : 5' - ctgcagcggccgctactagta cttgttgtcttgcagcggggtgctgccaagggcatcaagac - 3'

References