Difference between revisions of "Part:BBa K389402"

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<partinfo>BBa_K389402 short</partinfo>
 
<partinfo>BBa_K389402 short</partinfo>
  
This is a modified <partinfo>BBa_I746380</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 10 fold compared to a system without sensitivity tuner ([http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project#Results Cambridge, iGEM 2007, amplifier project]).  
+
This is a modified <partinfo>BBa_I746380</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10[luc2] vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 10 fold compared to a system without sensitivity tuner ([http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project#Results Cambridge, iGEM 2007, amplifier project]).  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:40, 17 October 2010

Sensitivity tuner + luciferase

This is a modified BBa_I746380 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10[luc2] vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 10 fold compared to a system without sensitivity tuner ([http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project#Results Cambridge, iGEM 2007, amplifier project]).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 2639
    Illegal NgoMIV site found at 2660
    Illegal AgeI site found at 818
    Illegal AgeI site found at 930
    Illegal AgeI site found at 1132
    Illegal AgeI site found at 2363
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2545