Difference between revisions of "Part:BBa K389403"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K389403 short</partinfo>
 
<partinfo>BBa_K389403 short</partinfo>
  
−
This is a modified <partinfo>BBa_I746390</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.
+
This is a modified <partinfo>BBa_I746390</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 30 - 35 fold compared to a system without sensitivity tuner ([http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project#Results Cambridge, iGEM 2007, amplifier project]).  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:20, 17 October 2010

Sensitivity tuner + luciferase

This is a modified BBa_I746390 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 30 - 35 fold compared to a system without sensitivity tuner ([http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project#Results Cambridge, iGEM 2007, amplifier project]).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 2666
    Illegal NgoMIV site found at 2687
    Illegal AgeI site found at 845
    Illegal AgeI site found at 957
    Illegal AgeI site found at 1159
    Illegal AgeI site found at 2390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2572