Difference between revisions of "Part:BBa K389401"

Revision as of 14:02, 17 October 2010

Sensitivity tuner + luciferase

This is a modified BBa_I746370 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 15 - 20 fold compared to a system without sensitivity tuner ([http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project#Results Cambridge, iGEM 2007, amplifier project]).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1295
Illegal NgoMIV site found at 2639
Illegal NgoMIV site found at 2660
Illegal AgeI site found at 818
Illegal AgeI site found at 930
Illegal AgeI site found at 1132
Illegal AgeI site found at 2363
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2545

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K389401 short</partinfo>
-This is a modified <partinfo>I746370</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 15 - 20 fold compared to a system without sensitivity tuner (Cambridge, iGEM 2007, amplifier project).
+This is a modified <partinfo>I746370</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 15 - 20 fold compared to a system without sensitivity tuner ([http://parts.mit.edu/igem07/index.php/Cambridge/Amplifier_project#Results Cambridge, iGEM 2007, amplifier project]).
 <!-- Add more about the biology of this part here