Difference between revisions of "Part:BBa K299025"

Revision as of 23:20, 16 October 2010

RBS measurement device pT7 J61100 GFP

RBS measurement device pT7 J61101 GFP

This part contains RBS J61100 from the Anderson's collection.

In the physical DNA after J61100 in composite parts there is Aactag sequence instead of the biobrick scar Tactag. J61100 was originally designed in a way that its last nucleotide "A" is also first nucleotide of the suffix (wchich should be T)- it modifies the suffix. The sequence of J61101 in the registry does not include its last nucleotide. If last nucleotide were included the registry sequence would show the additional nucleotide, now it shows T instead of A.

The seguence of the J612100 does not match its physical DNA

To go around this problem in out bricks we used scarless assembly option and manually included the scars:

BBa_K299800 DNA standard biobrick scar (if next part starts with ATG) BBa_K299801 DNA standard biobrick scar (if next sequence does NOT start with ATG) BBa_K299802 DNA Aactag - like a biobrick scar but first T changed to A BBa_K299803 DNA Gactag - like a biobrick scar but first T changed to G BBa_K299804 DNA Cactag - like a biobrick scar but first T changed to C.

This brick consists of the following subparts:

I719005[T7promoter] BBa_K299801[standard scar] BBa_J61100[RBS] BBa_K299802[Aactag] BBa_E0040[GFP] BBa_K299801[standard scar] BBa_B0010[terminator] BBa_K299801[standard scar] BBa_B0012[terminator]

Sequence and Features