Difference between revisions of "Part:BBa K389422"

Revision as of 16:06, 16 October 2010

VirA/G signaling system + sensitivity tuner + luciferase

This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.

Usage and Biology

To see, how this BioBrick works, watch the following animation:

Animation of the functionality of the BioBricks BBa_K389421, BBa_K389422 and BBa_K389423.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1632
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 5002
Illegal NgoMIV site found at 6346
Illegal NgoMIV site found at 6367
Illegal AgeI site found at 4525
Illegal AgeI site found at 4637
Illegal AgeI site found at 4839
Illegal AgeI site found at 6070
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1768
Illegal SapI.rc site found at 6252

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K389422 short</partinfo>
@@ Line 5: / Line 4: @@
 This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+To see, how this BioBrick works, watch the following animation:
+[[Image:IGEM_Bielefeld_K389421.gif‎|500px|thumb|center|Animation of the functionality of the BioBricks <partinfo>K389421</partinfo>, <partinfo>K389422</partinfo> and <partinfo>K389423</partinfo>.]]
 <!-- -->