Difference between revisions of "Part:BBa K412000:Design"

 
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<partinfo>BBa_K412000 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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We knew we wanted a nitrate sensitive promoter followed by a reporter. Therefore we digested PyeaR with SpeI and PstI and digested GFP with XbaI and PstI and then ligated the two parts together to form this composite part.
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We knew we wanted a nitrate sensitive promoter followed by a reporter to work in E.coli. Therefore we digested PyeaR with SpeI and PstI and digested GFP with XbaI and PstI and then ligated the two parts together to form this composite part.
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===Source===
 
===Source===

Latest revision as of 22:12, 15 October 2010

PyeaR+GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 770


Design Notes

We knew we wanted a nitrate sensitive promoter followed by a reporter to work in E.coli. Therefore we digested PyeaR with SpeI and PstI and digested GFP with XbaI and PstI and then ligated the two parts together to form this composite part.

Source

We got both subparts from the registry, when we looked for a nitrate detector and a reporter.

References