Difference between revisions of "Part:BBa K424018:Design"
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| + | Qinhong Wang, Xiangdong Fang, Baojun Bai, Xiaolin Liang, Patrick J. Shuler, William A. Goddard III, Yongchun Tang. 2007. Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery. Biotechnology and Bioengineering Volume 98, Issue 4, pages 842–853. | ||
Revision as of 00:18, 15 October 2010
Rhamnosiltransferase BioBrick (Rh1AB_BB)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 169
Illegal BamHI site found at 729
Illegal XhoI site found at 905
Illegal XhoI site found at 2191 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1084
Illegal NgoMIV site found at 1805
Illegal NgoMIV site found at 1918 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 394
Illegal BsaI site found at 1434
Illegal BsaI.rc site found at 578
Design Notes
The RhlAB gene complex have shown illegal restriction sites by the restriccition enzymes of the assembly standard protocol 10. We face this problem by applying the mutagenesis process that consist in doing silent mutation that change the nucleotides of our gene that are cutted by the restriction enzymes to made able the construction of our BioBrick.
Source
the genomic sequence of RhlAB was reviwed in genBank http://www.ncbi.nlm.nih.gov/nuccore/L28170.1
References
Qinhong Wang, Xiangdong Fang, Baojun Bai, Xiaolin Liang, Patrick J. Shuler, William A. Goddard III, Yongchun Tang. 2007. Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery. Biotechnology and Bioengineering Volume 98, Issue 4, pages 842–853.