Difference between revisions of "Part:BBa K329000"

Revision as of 16:29, 12 October 2010

Medium RBS (B0032) - Tetracycline resistance protein TetA(C) (J31007)

Tetracycline resistance protein (TetA(C) inner-membrane-associated protein BBa_J31007) with a Medium RBS upstream (BBa_B0032). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see BBa_K329003, BBa_K329004)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 163
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 309
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 335
Illegal NgoMIV site found at 703
Illegal NgoMIV site found at 863
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K329000 short</partinfo>
-Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Medium RBS upstream (<partinfo>BBa_B0032</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see <partinfo>BBa_K329003</partinfo>, <partinfo>BBa_K329004</partinfo>))
+Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Medium RBS upstream (<partinfo>BBa_B0032</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth. We have designed several translational units for TetA(C) (see <partinfo>BBa_K329003</partinfo>, <partinfo>BBa_K329004</partinfo>)
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