Difference between revisions of "Part:BBa K398402:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed. | + | Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed from the CDS. An extra XbaI site will be found between RBS and Promoter so you can increase the expression level of the protein by changing the promoter. |
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===Source=== | ===Source=== | ||
Latest revision as of 10:00, 12 October 2010
Prefoldin-alpha generator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed from the CDS. An extra XbaI site will be found between RBS and Promoter so you can increase the expression level of the protein by changing the promoter.
Source
The NCBI genomic sequence was in silico enhanced for iGEM purposes