Difference between revisions of "Part:BBa K330002"
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The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme from Escherichia coli. | The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme from Escherichia coli. | ||
| − | Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacteria. There are three often used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays | + | Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacteria. There are three often used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays vary (5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay, 4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl β-D-glucuronide for spectrophotometric assay). |
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Revision as of 14:10, 10 October 2010
gusA reporter gene
The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme from Escherichia coli.
Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacteria. There are three often used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays vary (5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay, 4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl β-D-glucuronide for spectrophotometric assay).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]