Difference between revisions of "Part:BBa K318500"
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<partinfo>BBa_K318500 short</partinfo>
 
<partinfo>BBa_K318500 short</partinfo>
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Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices: BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB).
 
Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices: BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB).
  
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RcsA is a transcription factor that is know to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsB, these two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis.
 
RcsA is a transcription factor that is know to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsB, these two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis.
 
See more on [[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA]]
 
See more on [[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA]]
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K318500 SequenceAndFeatures</partinfo>
 
  
 
===Characterization===
 
===Characterization===
 
Download Zip file HERE.
 
Download Zip file HERE.
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(TESTING Data HERE)
 
(TESTING Data HERE)
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K318500 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 22:48, 8 October 2010

lacI pL + RBS + RcsA + TT

Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices: BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB).

Usage and Biology

RcsA is a transcription factor that is know to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsB, these two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. See more on http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA

Characterization

Download Zip file HERE.

(TESTING Data HERE)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]