Difference between revisions of "Part:BBa K318500"

Revision as of 22:39, 8 October 2010

Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices: BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB).

Usage and Biology

RcsA is a transcription factor that is know to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsB, these two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. See more on http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
-<partinfo>BBa_K318500 short</partinfo>
+Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices: BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB).
-under construction
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+RcsA is a transcription factor that is know to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsB, these two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis.
+See more on [[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA]]
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K318500 SequenceAndFeatures</partinfo>