Difference between revisions of "Part:BBa K322237:Design"

 
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<partinfo>BBa_K322237 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Forward sequence looks good. Reverse sequence missing the base between the stop codon and SpeI site. This is also missing in the S284T clone (see below) suggesting that it is missing in the primer, or did we use a vector based primer? In which case it would suggest that it was missing in the original clone. Trivial but annoying.
 
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The suffix seems to be missing a base between the stop codon and the SpeI site. It seem this was missing in the original clone.
  
 
===Source===
 
===Source===

Latest revision as of 19:15, 7 October 2010

Firefly luciferase from Photinus pyralis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

The suffix seems to be missing a base between the stop codon and the SpeI site. It seem this was missing in the original clone.

Source

The origional sequence is from Photinus pyralis, a the gene here is derived from BioBrick BBa-I712019.

References