Difference between revisions of "Part:BBa K330000:Design"

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===Design Notes===
 
===Design Notes===
  
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FLAG-tag is a polypeptide protein tag which is commonly used in molecular biology, particularly recombinant DNA technology. It can be added to the N-terminal, C-terminal or within a protein coding sequence. FLAG-tag is used in affinity chromatography to separate or detect recombinant, overexpressed protein from wild-type protein; it can also be used in cellular localization studies by immunofluorescence or detection by SDS-PAGE protein electrophoresis.
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FLAG-tag is a polypeptide protein tag which is commonly used in molecular biology, particularly recombinant DNA technology. It can be added to the N-terminal or C-terminal of a protein coding sequence; it can also be inserted within two domains of a chimeric protein, giving that that the disruption of FLAG-tag will not affect the function of the polypeptide. FLAG-tag is used in affinity chromatography to separate or detect recombinant, overexpressed protein from wild-type protein; it can also be used in cellular localization studies by immunofluorescence or detection by SDS-PAGE protein electrophoresis.
  
 
===Source===
 
===Source===

Revision as of 14:15, 7 October 2010

Protein Tag: FLAG-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FLAG-tag is a polypeptide protein tag which is commonly used in molecular biology, particularly recombinant DNA technology. It can be added to the N-terminal or C-terminal of a protein coding sequence; it can also be inserted within two domains of a chimeric protein, giving that that the disruption of FLAG-tag will not affect the function of the polypeptide. FLAG-tag is used in affinity chromatography to separate or detect recombinant, overexpressed protein from wild-type protein; it can also be used in cellular localization studies by immunofluorescence or detection by SDS-PAGE protein electrophoresis.

Source

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References