Difference between revisions of "Part:BBa K389012:Design"

(Source)
(Design Notes)
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
 
* medium strong constitutive promoter
 
* medium strong constitutive promoter
* double terminator (forward) to keep basal expression of kanamycin resistance low  
+
* double terminator (forward) to keep basal expression of luciferase low  
 
** double terminator <partinfo>BBa_B0017</partinfo> instead of <partinfo>BBa_B0015</partinfo> because of problems mentioned with <partinfo>BBa_B0012</partinfo>
 
** double terminator <partinfo>BBa_B0017</partinfo> instead of <partinfo>BBa_B0015</partinfo> because of problems mentioned with <partinfo>BBa_B0012</partinfo>
 
* luciferase gene to show the activity of the ''vir'' promoter
 
* luciferase gene to show the activity of the ''vir'' promoter

Revision as of 12:43, 7 October 2010

VirA reporter system luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
    Illegal NgoMIV site found at 2539
    Illegal NgoMIV site found at 2560
    Illegal AgeI site found at 2263
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2445


Design Notes

  • medium strong constitutive promoter
  • double terminator (forward) to keep basal expression of luciferase low
  • luciferase gene to show the activity of the vir promoter

Source

References