Difference between revisions of "Part:BBa K389012:Design"
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===Source=== | ===Source=== | ||
| − | + | * ''virG'' gene synthesized by Mr. Gene (<partinfo>BBa_K389002</partinfo>) | |
| + | * ''vir'' promoter from ''A. tumefaciens'' C58 (<partinfo>BBa_K389003</partinfo>) | ||
| + | * luciferase gene from Promega's pGL4.10luc2 vector (<partinfo>K389004</partinfo>) | ||
| + | * constitutive promoter and double terminator from parts.igem (<partinfo>BBa_J23110</partinfo>, <partinfo>BBa_B0017</partinfo>) | ||
===References=== | ===References=== | ||
Revision as of 12:41, 7 October 2010
VirA reporter system luc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1195
Illegal NgoMIV site found at 2539
Illegal NgoMIV site found at 2560
Illegal AgeI site found at 2263 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2445
Design Notes
- medium strong constitutive promoter
- double terminator (forward) to keep basal expression of kanamycin resistance low
- luciferase gene to show the activity of the vir promoter
Source
- virG gene synthesized by Mr. Gene (BBa_K389002)
- vir promoter from A. tumefaciens C58 (BBa_K389003)
- luciferase gene from Promega's pGL4.10luc2 vector (BBa_K389004)
- constitutive promoter and double terminator from parts.igem (BBa_J23110, BBa_B0017)