Difference between revisions of "Part:BBa K389012:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K389012 short</partinfo>
 
<partinfo>BBa_K389012 short</partinfo>
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===Design Notes===
 
===Design Notes===
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medium strong constitutive promotor, terminator to keep basal expression of luciferase low
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* medium strong constitutive promoter
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+
* double terminator (forward) to keep basal expression of kanamycin resistance low  
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+
** double terminator <partinfo>BBa_B0017</partinfo> instead of <partinfo>BBa_B0015</partinfo> because of problems mentioned with <partinfo>BBa_B0012</partinfo>
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* luciferase gene to show the activity of the ''vir'' promoter
  
 
===Source===
 
===Source===

Revision as of 12:39, 7 October 2010

VirA reporter system luc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
    Illegal NgoMIV site found at 2539
    Illegal NgoMIV site found at 2560
    Illegal AgeI site found at 2263
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2445


Design Notes

  • medium strong constitutive promoter
  • double terminator (forward) to keep basal expression of kanamycin resistance low
  • luciferase gene to show the activity of the vir promoter

Source

parts.igem, synthetic gene by Mr. Gene, pGL4.10luc2 by Promega, TI-plasmid from Agrobacterium tumefaciens C58

References