Difference between revisions of "Part:BBa K389011:Design"
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* ''virG'' gene synthesized by Mr. Gene (<partinfo>K389002</partinfo>) | * ''virG'' gene synthesized by Mr. Gene (<partinfo>K389002</partinfo>) | ||
| − | * ''vir'' promoter from ''A. tumefaciens'' C58 (partinfo>K389003</partinfo>) | + | * ''vir'' promoter from ''A. tumefaciens'' C58 (<partinfo>K389003</partinfo>) |
| + | * ''kanR'' gene made from BioBrick <partinfo>P1003</partinfo> | ||
* constitutive promoter and double terminator from parts.igem (<partinfo>J23110</partinfo>, <partinfo>B0017</partinfo>) | * constitutive promoter and double terminator from parts.igem (<partinfo>J23110</partinfo>, <partinfo>B0017</partinfo>) | ||
===References=== | ===References=== | ||
Revision as of 12:34, 7 October 2010
VirA screening device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- medium strong constitutive promoter,
- double terminator (forward) to keep basal expression of kanamycin resistance low
- kanamycin resistance for screening with plates
Source
- virG gene synthesized by Mr. Gene (BBa_K389002)
- vir promoter from A. tumefaciens C58 (BBa_K389003)
- kanR gene made from BioBrick BBa_P1003
- constitutive promoter and double terminator from parts.igem (BBa_J23110, BBa_B0017)