Difference between revisions of "Part:BBa K389002:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
point mutations G56V and I77V so it works without ''rpoA'' gene from ''Agrobacterium tumefaciens'' in ''Escherichia coli'' (YC Jung ''et. al.'', 2004), synthesized by Mr. Gene, so optimated codon usage for ''E. coli'' and removal of illegal restriction sites
+
* point mutations G56V and I77V so it works without ''rpoA'' gene from ''Agrobacterium tumefaciens'' in ''Escherichia coli'' (YC Jung ''et. al.'', 2004)   
 +
* synthesized by Mr. Gene, so:
 +
** optimated codon usage for ''E. coli''  
 +
** removal of illegal restriction sites
  
 
===Source===
 
===Source===

Revision as of 14:47, 5 October 2010

Mutated virG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • point mutations G56V and I77V so it works without rpoA gene from Agrobacterium tumefaciens in Escherichia coli (YC Jung et. al., 2004)
  • synthesized by Mr. Gene, so:
    • optimated codon usage for E. coli
    • removal of illegal restriction sites

Source

synthetic

References

YC Jung et al. (2004) Mutants of Agrobacterium tumefaciens virG Gene That Activate Transcription of vir Promoter in Escherichia coli, Current Microbiol 49:334-340.