Difference between revisions of "Part:BBa K395702"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K395702 short</partinfo>
 
<partinfo>BBa_K395702 short</partinfo>
  
This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when added beta-carotene as the substrate.
+
This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.
It is necessary to add beta-carotene or beta-carotene synthesis operon if you want to get zeaxanthin by using this part.
+
It is necessary to add either beta-carotene or the synthesis operon.  
I used this part with beta-carotene synthesis operon (BBa_K274210) as double plasmid in the project.
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:09, 4 October 2010

rbs+crtZ under pBad promoter for double plasmids

This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. It is necessary to add either beta-carotene or the synthesis operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961