Difference between revisions of "Part:BBa K302017"

Latest revision as of 15:15, 28 September 2010

spaIFEG cluster

Coding sequences and RBS' for spaIFEG cluster.

Subtilin is a lantibiotic peptide produced naturally by Bacillus subtilis ATCC 6633 but not by Bacillus subtilis 168.

spaI encodes a membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores.

spaFEG encode three proteins which form a membrane transporter that removes subtilin from the membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 740
Illegal BglII site found at 1488
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1755
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1035
Illegal SapI site found at 1315
Illegal SapI.rc site found at 2038

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K302017 short</partinfo>
-Coding sequences and RBS' for spaIFEG cluster.
+Coding sequences and RBS' for ''spaIFEG'' cluster.
-SpaI &mdash; Membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores.
-SpaFEG &mdash; three proteins from B. Subtilis which form a membrane transporter that removes subtilin from the membrane, preventing it from forming pores and killing the cells.
+Subtilin is a lantibiotic peptide produced naturally by ''Bacillus subtilis'' ATCC 6633 but not by ''Bacillus subtilis'' 168.
+''spaI'' encodes a membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores.
+''spaFEG'' encode three proteins which form a membrane transporter that removes subtilin from the membrane.