Difference between revisions of "Part:BBa K302018"

 
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<partinfo>BBa_K302018 short</partinfo>
 
<partinfo>BBa_K302018 short</partinfo>
  
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Coding sequences for spaIFEG cluster. Device uses Pveg/spoVG promoter/RBS (BBa_K143053) and double terminator (BBa_B0014). This device will confer immunity to subtilin lantibiotic.
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Subtilin is a lantibiotic peptide produced naturally by ''Bacillus subtilis'' ATCC 6633 but not by ''Bacillus subtilis'' 168. This part encodes two separate subtilin immunity systems, which together provide a high degree of subtilin resistance.  
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Revision as of 14:24, 28 September 2010

Subtilin immunity

Subtilin is a lantibiotic peptide produced naturally by Bacillus subtilis ATCC 6633 but not by Bacillus subtilis 168. This part encodes two separate subtilin immunity systems, which together provide a high degree of subtilin resistance.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 837
    Illegal BglII site found at 1585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1132
    Illegal SapI site found at 1412
    Illegal SapI.rc site found at 2135