Difference between revisions of "Part:BBa K302016"

Latest revision as of 14:18, 28 September 2010

Swarming

Bacillus subtilis 168 is unable to swarm on a solid surface e.g., agar plate or concrete. This is due to two issues:

Strain 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids the bacteria to swarm on a solid or semi solid medium. This is because the 168 chromosome contains a frameshift mutation in the gene sfp.
Strain 168 is also unable to bio-synthesize flagella. In the undomesticated strain 3610, the gene swrA helps in the bio-synthesis of flagella. The laboratory strain 168 again contains a frameshift mutation in swrA and thus the strain moves on the surface at a very slow pace.

This part restores the swarming ability of Bacillus subtilis 168.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 774
Illegal SapI.rc site found at 784

@@ Line 3: / Line 3: @@
 ''Bacillus subtilis'' 168 is unable to swarm on a solid surface e.g., agar plate or concrete. This is due to two issues:
-# Strain 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids the bacteria to swarm on a solid or semi solid medium. This is because the 168 chromosome contains a frameshift mutation in the gene sfp.
+# Strain 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids the bacteria to swarm on a solid or semi solid medium. This is because the 168 chromosome contains a frameshift mutation in the gene ''sfp''.
-# Strain 168 is also unable to bio-synthesize flagella. In the undomesticated strain 3610, the gene swrA helps in the bio-synthesis of flagella. The laboratory strain 168 again contains a frameshift mutation in the gene swrA and thus the strain moves on the surface at a very slow pace.
+# Strain 168 is also unable to bio-synthesize flagella. In the undomesticated strain 3610, the gene ''swrA'' helps in the bio-synthesis of flagella. The laboratory strain 168 again contains a frameshift mutation in ''swrA'' and thus the strain moves on the surface at a very slow pace.
 This part restores the swarming ability of ''Bacillus subtilis'' 168.