Difference between revisions of "Part:BBa K302016"
Line 7: Line 7:
  
 
This part restores the swarming ability of ''Bacillus subtilis'' 168.
 
This part restores the swarming ability of ''Bacillus subtilis'' 168.
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:13, 28 September 2010

Swarming

Bacillus subtilis 168 is unable to swarm on a solid surface e.g., agar plate or concrete. This is due to two issues:

  1. Strain 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids the bacteria to swarm on a solid or semi solid medium. This is because the 168 chromosome contains a frameshift mutation in the gene sfp.
  2. Strain 168 is also unable to bio-synthesize flagella. In the undomesticated strain 3610, the gene swrA helps in the bio-synthesis of flagella. The laboratory strain 168 again contains a frameshift mutation in the gene swrA and thus the strain moves on the surface at a very slow pace.

This part restores the swarming ability of Bacillus subtilis 168.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 774
    Illegal SapI.rc site found at 784