Difference between revisions of "Part:BBa K302016"

Revision as of 14:13, 28 September 2010

Swarming

Bacillus subtilis 168 is unable to swarm on a solid surface e.g., agar plate or concrete. This is due to two issues:

Strain 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids the bacteria to swarm on a solid or semi solid medium. This is because the 168 chromosome contains a frameshift mutation in the gene sfp.
Strain 168 is also unable to bio-synthesize flagella. In the undomesticated strain 3610, the gene swrA helps in the bio-synthesis of flagella. The laboratory strain 168 again contains a frameshift mutation in the gene swrA and thus the strain moves on the surface at a very slow pace.

This part restores the swarming ability of Bacillus subtilis 168.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 774
Illegal SapI.rc site found at 784

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 __NOTOC__
 <partinfo>BBa_K302016 short</partinfo>
-Swarming
+''Bacillus subtilis'' 168 is unable to swarm on a solid surface e.g., agar plate or concrete. This is due to two issues:
+# Strain 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids the bacteria to swarm on a solid or semi solid medium. This is because the 168 chromosome contains a frameshift mutation in the gene sfp.
+# Strain 168 is also unable to bio-synthesize flagella. In the undomesticated strain 3610, the gene swrA helps in the bio-synthesis of flagella. The laboratory strain 168 again contains a frameshift mutation in the gene swrA and thus the strain moves on the surface at a very slow pace.
+This part restores the swarming ability of ''Bacillus subtilis'' 168.
 <!-- Add more about the biology of this part here