Difference between revisions of "Part:pSB1A10:Design"
Line 1: Line 1:
 
The process for building pSB1A10,was:
 
The process for building pSB1A10,was:
 
#Build [[Part:BBa_I13534|I13534]] using normal BB construcion.
 
#Build [[Part:BBa_I13534|I13534]] using normal BB construcion.
−
#Insert [[Part:BB_aI13534|I13534]] into psb1A3 plasmid
+
#Insert [[Part:BBa_I13534|I13534]] into psb1A3 plasmid
 
#Scar out the BB prefix and suffix using [[Part:BBa_I13450]]
 
#Scar out the BB prefix and suffix using [[Part:BBa_I13450]]
 
#Re-insert a BB prefix/suffix into the center of [[Part:BBa_I13534|I13534]] using primers to the "insertion region" [[Part:BBa_I13457|I13457]] of and around-the-horn PCR.
 
#Re-insert a BB prefix/suffix into the center of [[Part:BBa_I13534|I13534]] using primers to the "insertion region" [[Part:BBa_I13457|I13457]] of and around-the-horn PCR.
  
 
Some design notes:
 
Some design notes:
−
#The inclusion of the 'barcode' was unintentional (it was attached to [[Part:E1010]]), it has no (intended) function.
+
#The inclusion of the 'barcode' was unintentional (it was attached to [[Part:BBa_E1010]]), it has no (intended) function.
 
#The "spacer artifacts" at position 22 and 5225 are artifacts from around-the-horn PCR for inserting the BB restriction sites into the center of the plasmid.  If you look at [[Part:BBa_I13457]] you will see the corresponding regions in the section annotated "insertion region".
 
#The "spacer artifacts" at position 22 and 5225 are artifacts from around-the-horn PCR for inserting the BB restriction sites into the center of the plasmid.  If you look at [[Part:BBa_I13457]] you will see the corresponding regions in the section annotated "insertion region".

Revision as of 08:00, 26 May 2006

The process for building pSB1A10,was:

  1. Build I13534 using normal BB construcion.
  2. Insert I13534 into psb1A3 plasmid
  3. Scar out the BB prefix and suffix using Part:BBa_I13450
  4. Re-insert a BB prefix/suffix into the center of I13534 using primers to the "insertion region" I13457 of and around-the-horn PCR.

Some design notes:

  1. The inclusion of the 'barcode' was unintentional (it was attached to Part:BBa_E1010), it has no (intended) function.
  2. The "spacer artifacts" at position 22 and 5225 are artifacts from around-the-horn PCR for inserting the BB restriction sites into the center of the plasmid. If you look at Part:BBa_I13457 you will see the corresponding regions in the section annotated "insertion region".