|
|
| Line 1: |
Line 1: |
| − | <div style="font-family: Arial; padding: 00px; width: 680px; border: 0px solid #000000;">
| + | __NOTOC__ |
| − | {{Template:K325219 page header}}
| + | <partinfo>BBa_K325219 short</partinfo> |
| − | {{Template:K325219 Characterization Navbar}}
| + | |
| − | <div style="padding: 00px; width: 680px; border: 0px solid #000000;">
| + | |
| − | '''Description'''<br>
| + | |
| − | A transcription factor, LuxR (''<partinfo> C0062</partinfo> '') that is active in the presence of cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a TetR (''<partinfo>BBa_C0040</partinfo>'') regulated operator (''<partinfo>BBa_R0040</partinfo>''). ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]]. ''Device output'' is [[PoPS]] from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function. Get the pdf datasheet [[:Image:EndyFig1-F2620DataSheet.pdf|here]]. | + | |
| | | | |
| | + | <partinfo>BBa_K325219 SequenceAndFeatures</partinfo> |
| | | | |
| − | '''Performance'''<br>
| + | |
| − | <center>
| + | ===Design Notes=== |
| − | {|{{Table}}
| + | Biobrick assembly |
| − | !Experiment<sup>1</sup>
| + | |
| − | !Characteristic<sup>1</sup>
| + | |
| − | !Value<sup>1</sup>
| + | |
| − | |-
| + | ===Source=== |
| − | |rowspan="3"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]
| + | |
| − | |''Maximum Output''
| + | Biobrick assembly from its two subparts |
| − | |6.6 [[PoPS]] cell<sup>-1</sup>
| + | |
| − | |-
| + | ===References=== |
| − | |''Hill coefficient''
| + | |
| − | |1.6
| + | |
| − | |-
| + | |
| − | |[[Switch Point|''Switch Point'']]
| + | |
| − | |1.5E-9 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
| + | |
| − | |-
| + | |
| − | |[[Part:BBa_F2620:Response time|'''Response time:''']]
| + | |
| − | |<1 min
| + | |
| − | |-
| + | |
| − | |rowspan="2"|[[Part:BBa_F2620:Specificity|'''Input compatibility''']]
| + | |
| − | |''Strong response to''
| + | |
| − | |[[3OC6HSL|3OC<sub>6</sub>HSL]], C<sub>6</sub>HSL , C<sub>7</sub>HSL, 3OC<sub>8</sub>HSL, C<sub>8</sub>HSL
| + | |
| − | |-
| + | |
| − | |''Weak response to''
| + | |
| − | |C<sub>4</sub>HSL, C<sub>10</sub>HSL, C<sub>12</sub>HSL
| + | |
| − | |-
| + | |
| − | |rowspan="2"|[[Part:BBa_F2620:Stability|'''Stability''']]
| + | |
| − | |[[Genetic Stability|''Genetic Stability'']]<br>(Low/High Input)
| + | |
| − | |>92/>56 generations
| + | |
| − | |-
| + | |
| − | |[[Performance Stability|''Performance Stability'']]<br>(Low/High Input)
| + | |
| − | |>92/>56 generations
| + | |
| − | |-
| + | |
| − | |rowspan="4"|Demand
| + | |
| − | |rowspan="1"|Internal Demand<br>(Low/High Input)
| + | |
| − | |Not measured
| + | |
| − | |-
| + | |
| − | |rowspan="2"|[[Transcription Demand|''Transcriptional output demand:'']]<br>(Low/High Input)<br>Nt = length of downstream transcript in nucleotides
| + | |
| − | |(0/6xNt) nucleotides cell<sup>-1</sup> s<sup>-1</sup>
| + | |
| − | |-
| + | |
| − | |(0/1.5E-1xNt) RNAP cell<sup>-1</sup>
| + | |
| − | |-
| + | |
| − | |[[Growth Rate|''Growth Rate'']]<br>(Low/High Input)
| + | |
| − | |54/59 min Doubling time
| + | |
| − | |}
| + | |
| − | </center>
| + | |
| − | <sup>1</sup>Measured by Ania Labno and Barry Canton 2006-2007
| + | |
| − | <div style="padding: 00px; width: 680px">
| + | |
| − | '''Compatibility'''<br>
| + | |
| − | [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''<partinfo>BBa_V1000</partinfo>'',''<partinfo>BBa_V1001</partinfo>'',''<partinfo>BBa_V1002</partinfo>'', [[Chassis/Cell-Free_Systems/Commercial_E.coli_S30| E.Coli S30 Extract]] [[Chassis/Cell-Free_Systems/Commercial_E.coli_S30/F2620 | (data)]]<br>
| + | |
| − | [[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB3k3</partinfo>'' and ''<partinfo>pSB1A3</partinfo>''<br>
| + | |
| − | [[Part Types|''Devices:'']] Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'', ''<partinfo>BBa_E0434</partinfo>'', ''<partinfo>BBa_E0240</partinfo>''<br>
| + | |
| − | Crosstalk with systems containing ''<partinfo>BBa_C0040</partinfo>''.<br>
| + | |
| − | [[Help:Signalling|''Cell-Cell Signaling Systems:'']] Crosstalk with devices using [[3OC6HSL|3OC<sub>6</sub>HSL]], C6HSL, C7HSL, C8HSL, C10HSL.
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
