Difference between revisions of "Part:BBa K325219"

 
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<partinfo>BBa_K325219 short</partinfo>
 
<partinfo>BBa_K325219 short</partinfo>
  
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This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata). It also generates the luciferin regenerating enzyme. When luciferin and D-cysteine are added this BioBrick should give sustained light output.  With simply luciferin it will give a burst of light output.
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This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter.
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D-Luciferin has to be added to obtain light output.
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Revision as of 09:50, 24 September 2010

Red Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)

This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. D-Luciferin has to be added to obtain light output.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2118
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1550
    Illegal SapI.rc site found at 3334