Difference between revisions of "Part:BBa K385002:Experience"
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===Applications of BBa_K385002=== | ===Applications of BBa_K385002=== | ||
| + | The MS2 coat protein reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast in the single copy shuttle vector pRS415. | ||
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| + | The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed usin a fluorescence microscope optimised for GFP visualisation (Figure 1). | ||
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| + | A control culture of the same transformant was grown using glucose as the carbon source; these conditions do not activate the GAL promoter. The results (Figure 2) show no GFP fluorescence. | ||
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| + | Overall the results indicate that the MS2 coat protein can be successfully expressed as a protein fusion with other standard parts. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:24, 22 September 2010
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Please enter
how you used this part and how it worked out.
Applications of BBa_K385002
The MS2 coat protein reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast in the single copy shuttle vector pRS415.
The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed usin a fluorescence microscope optimised for GFP visualisation (Figure 1).
A control culture of the same transformant was grown using glucose as the carbon source; these conditions do not activate the GAL promoter. The results (Figure 2) show no GFP fluorescence.
Overall the results indicate that the MS2 coat protein can be successfully expressed as a protein fusion with other standard parts.
User Reviews
UNIQb314912cd77450af-partinfo-00000000-QINU UNIQb314912cd77450af-partinfo-00000001-QINU