Difference between revisions of "Part:BBa K318030:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Note that the constitutive promoter is oriented to express whatever gene is downstream of the final hixC site. | + | Note that the constitutive promoter is oriented to express whatever gene is downstream of the final hixC site after recombination. |
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===Source=== | ===Source=== | ||
Revision as of 16:05, 27 August 2010
lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 43
Illegal NheI site found at 66 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 162
Design Notes
Note that the constitutive promoter is oriented to express whatever gene is downstream of the final hixC site after recombination.
Source
From Synthesis