Difference between revisions of "Part:BBa K367012:Design"
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<partinfo>BBa_K367012 short</partinfo> | <partinfo>BBa_K367012 short</partinfo> | ||
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+ | <p>''fecI'' and ''fecR'' genes of ''Escherichia coli'', encoding an ECF sigma factor and a membrane signal transduction protein respectively, involved in signal transduction of the ferric citrate-dependent Fec system. They have been synthesized in the same Biobrick because of their 4 bp overlap in the ''E. coli'' genome, not to reduce their expression. FecR protein interacts with the outer membrane sensor FecA and activates the sigma factor FecI, which acts over PfecA promoter region.</p> | ||
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<partinfo>BBa_K367012 SequenceAndFeatures</partinfo> | <partinfo>BBa_K367012 SequenceAndFeatures</partinfo> |
Revision as of 18:01, 26 August 2010
fecI & fecR overlapping coding sequences
fecI and fecR genes of Escherichia coli, encoding an ECF sigma factor and a membrane signal transduction protein respectively, involved in signal transduction of the ferric citrate-dependent Fec system. They have been synthesized in the same Biobrick because of their 4 bp overlap in the E. coli genome, not to reduce their expression. FecR protein interacts with the outer membrane sensor FecA and activates the sigma factor FecI, which acts over PfecA promoter region.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 322
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 325
Design Notes
Stop codon substituted by standard biobrick TAATAA double stop. PstI site at 1184 removed: CTGCAG to CTGCAA.
Source
This part has been made by PCR and SDM using E. coli K12 genome as template.