Difference between revisions of "Part:BBa K249019:Experience"

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When sequencing the stock we had from 2009 our team (Lethbridge) found a RBS mutation.  We attempted to overexpress the protein and found that it would not.  We do not know if this is the same for the kits that were sent out this year or not.  We recommend that you sequence the part before use and if it has the same mutation do not use directly but mutate the mutation out.  The mutation we found is as follows:
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There is not a sequencing error in the RBS but so far we have still not successfully overexpressed Mms6.
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              Mms6 7 VF Prefix  TCCCGACTGGAAAGCGGG-NNANAGNGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
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Mms6 7 VR A-Suffix (reversed)  TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
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              Mms6 8 VF Prefix  TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
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Mms6 8 VR A-Suffix (reversed)  TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
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                          mRBS  TCAC-ACAGGAAAG----------------------------------------------
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Revision as of 16:35, 17 August 2010

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Please enter how you used this part and how it worked out.

Applications of BBa_K249019

User Reviews

UNIQ62236dce8efd2fc9-partinfo-00000000-QINU UNIQ62236dce8efd2fc9-partinfo-00000001-QINU

There is not a sequencing error in the RBS but so far we have still not successfully overexpressed Mms6.