Difference between revisions of "Part:BBa J70626"

Latest revision as of 14:32, 11 August 2010

RFC12 His tagged RFP

This is a N-terminal fusion of a his tag onto a RFP protein, with the requisite stop codons. This part will cause cells to grow red, but if you are looking for a His-tagged RFP without the stop codons (i.e. for fusions), this part will not work. Either use BBa_J70595 (GFP), BBa_J70609, or some combination of the two if you wish to have a GFP and His tag respectively.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 587
Illegal AgeI site found at 699
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_J70626 short</partinfo>
-This is a N-terminal fusion of a his tag onto a GFP protein.  This part will cause cells to grow green, but if you are looking for a His-tagged GFP without the stop codons (i.e. for fusions), this part will not work.  Either use <partinfo>BBa_J70595</partinfo> (GFP), <partinfo>BBa_J70609</partinfo>, or some combination of the two if you wish to have a GFP and His tag respectively.
+This is a N-terminal fusion of a his tag onto a RFP protein, with the requisite stop codons.  This part will cause cells to grow  red, but if you are looking for a His-tagged RFP without the stop codons (i.e. for fusions), this part will not work.  Either use <partinfo>BBa_J70595</partinfo> (GFP), <partinfo>BBa_J70609</partinfo>, or some combination of the two if you wish to have a GFP and His tag respectively.
 <!-- Add more about the biology of this part here