Difference between revisions of "Part:BBa K299300"

Latest revision as of 22:59, 9 August 2010

J23100[promoter].K299107[TxbaIG].J61100[RBS] No b-brick scars between

J23100[promoter].K299107[xbaIG].J61101[RBS] = Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.

Also be careful part J61100 is in registry as AAAGAGGGGACA, and automattically when the B-brick suffix is added the next nucleotide generated by the registry page is 'T'. However the CORRECT J61101 sequence is:

AAAGAGGGGACA**a** (and then comes the rest of the suffix: actagt)

It means that the physical sequence is different to the record in registry for J61100, currently there is no way around it, because registry does not alow changes in suffix, and generates T anyway.

This part is just to enable automatic assembly of the Anderson's RBS collection in the original vector with parts cloned behind RBS. Anderson RBSes comes on PSB1A2 with additional promoter followed by T' XbaI G site and G. After this sequence the RBS is placed.

The original Anderson RBSes and vector are described here.

https://parts.igem.org/wiki/index.php?title=Part:BBa_J61100

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 37
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 37
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 37
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K299300 short</partinfo>
@@ Line 6: / Line 5: @@
 Nucleotide sequence: T+xbaI+G 'binds' promoter and RBS instead of the biobrick scar.
-Also be careful part J61100 is in registry as AAAGAGGGGACAA, and automattically when the B-brick suffix is added the next nucleotide generated by the registry page is 'T'. However the CORRECT J61101 sequence is:
+Also be careful part J61100 is in registry as AAAGAGGGGACA, and automattically when the B-brick suffix is added the next nucleotide generated by the registry page is 'T'. However the CORRECT J61101 sequence is:
-AAAGAGGGGACAA**a** (and then comes the rest of the suffix: ctagt)
+AAAGAGGGGACA**a** (and then comes the rest of the suffix: actagt)
 It means that the physical sequence is different to the record in registry for J61100, currently there is no way around it, because registry does not alow changes in suffix, and generates T anyway.