Difference between revisions of "Part:BBa J70620"

 
 
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<partinfo>BBa_J70620 short</partinfo>
 
<partinfo>BBa_J70620 short</partinfo>
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MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.
 
MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.
  
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===Usage and Biology===
 
===Usage and Biology===
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Common tag information:
  
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<ul>
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<li>Matrix: Cross-linked Amylose</li>
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<li>Yield/Purity: High yield, >70% purity</li>
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<li># Steps: One step</li>
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<li>Fusion Location: Internal</li>
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<li>Affects Function?: Possibly</li>
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</ul>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 17:06, 9 August 2010

RFC12 Maltose Binding Protein Internal Domain [malE]

This Maltose-binding protein is designed for either N-terminal or C-terminal fusions. MBP fused proteins can be purified in one step affinity chromatography using a cross-linked amylose matrix and then eluted with 10 mM maltose. Note that the amylose matrix can only be regenerated, in most cases, up to five times. The large size of this tag can also affect the fused protein.

MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.

Usage and Biology

Common tag information:

  • Matrix: Cross-linked Amylose
  • Yield/Purity: High yield, >70% purity
  • # Steps: One step
  • Fusion Location: Internal
  • Affects Function?: Possibly

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 55