Difference between revisions of "Part:BBa J70623"
m (→Usage and Biology) |
|||
| Line 11: | Line 11: | ||
<li>Protein Sequence: EQKLISEEDL </li> | <li>Protein Sequence: EQKLISEEDL </li> | ||
<li>Matrix: Monoclonal Antibody</li> | <li>Matrix: Monoclonal Antibody</li> | ||
| − | <li>Yield/Purity: Fairly low yields | + | <li>Yield/Purity: Fairly low yields</li> |
<li># Steps: One step</li> | <li># Steps: One step</li> | ||
<li>Fusion Location: C-terminus (N-terminus is <partinfo>J70622</partinfo>)</li> | <li>Fusion Location: C-terminus (N-terminus is <partinfo>J70622</partinfo>)</li> | ||
Latest revision as of 15:42, 9 August 2010
RFC12 cMyc Tag Tail Domain
This is a RFC12 compatible version of BBa_T2006. It is a cMyc tag tail domain meant for c-terminal fusions.
Usage and Biology
cMyc tags are commonly used for detection purposes but not usually purity due to the fact that the matrix cannot be easily regenerated, the low pH elution may affect the product, and the non-specificity of the antibodies used can mean impure product.
Common tag information:
- Protein Sequence: EQKLISEEDL
- Matrix: Monoclonal Antibody
- Yield/Purity: Fairly low yields
- # Steps: One step
- Fusion Location: C-terminus (N-terminus is BBa_J70622)
- Affects Function?: Tag will likely not, but the low pH elution may
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]